The Enzymatic Portal:

Tutorial Outline For Completing Protein and Enzyme Esterase Assays

Portal Outline I. Preview: The Unknowns II. Preparation A. For Protein Esterase Assay 1. The Buffer 2. The Standards 3. The Chromotophore Dye   B. For Enzyme Esterase Assay 1. The Buffer 2. The Standards 3. The Substrate 4. The Stop Solution

III. Execution A. Insertion- addition to spectrophotometric plate (Assays are done on separate plates. Directions for Protein Assay on top and for Enzyme Assay below pictures) 1. The Blanks 2. The Standards 3. The Buffer 4. The Unknowns 5. The Chromotophore Dye *For Protein Esterase Assay ONLY 6. The Substrate *For Enzyme Esterase Assay ONLY 7. Charts of Assays   B. Incubation and Termination *For Enzyme Esterase Assay ONLY

IV. Conclusion A. Using the Spectrophotometer   B. Calculations and Graphing Results (To Be Developed...) 1. Unknown Concentrations 2. Enzymatic Activity 3. Evaluate Data in a Report       Return to The Enzymatic Portal

     Enzyme Assay Summary                      Return to CESC