Introduction
The purpose of this web page is to provide the student with a simplified entry into the world of esterase assays using basic explanations and pictorial representations. Both the protein and enzyme esterase assays are explored in determining enzymatic activity, providing introductory knowledge into cellular activity and biology. The example chosen throughout this tutorial was derived from a lab project in the class Cell Biology given at Montreat College. It was completed as part of a study on the enzymatic activity of Mountain Laurel and Rhododendron trees in the vicinity of Black Mountain, NC. This tutorial was produced as part of an additional research project by Matthew J. Krip. The tutorial is branched into four sections under the portal outline as seen below. Click on the link to begin.
General Esterase [1-Naphthyl acetate] Activity Assay - The basic protocol was modified from the Sparks, T.C., W.S.Willis, H.H. Shorey &B.D. Hammock. 1979. Haemolymph juvenile hormone esterase activity in synchronous last instar larvae of the cabbage looper, Trichoplusia ni. J. Insect Physiol. 25: 125-132., Dr. Michael Roe's laboratory modification of this methodand utilized in the work for Mark Lassiter publications: Lassiter et. al (1994), Lassiter et. al. (1995), and Lassiter et. al. (1996). Assay modified for plant metabolism in Lassiter's lab - 2003.
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