Soaking the Probes:
Soaking the probes:
Each ISE probe must be soaked for at least thirty minutes before they are calibrated and used for taking measurements. Each probe has its own set of standard solutions, both a high standard and a low standard. The probes are soaked for thirty minutes in the high standard solution.
Calcuim ISE probe:
Locate the small plastic bottle labeled with blue tape. Rinse the bottle with distilled water. Fill the bottle three-quarters full with the Calcium high standard solution, also labeled with blue tape. Place the probe in this bottle, so that the tip of the probe does not touch the bottom, but so that the white dots (located just above the black rubber tip on the end of the probe) are completely submerged in the standard solution. Allow the probe to remain like this for at least thirty minutes.
Ammonium ISE probe:
Locate the small plastic bottle labeled with yellow tape. Rinse the bottle with distilled water. Fill the bottle three-quarters full with the Ammonium high standard solution, also labeled with yellow tape. Place the probe in this bottle, so that the tip of the probe does not touch the bottom, but so that the white dots (located just above the black rubber tip on the end of the probe) are completely submerged in the standard solution. Allow the probe to remain like this for at least thirty minutes.
Nitrate ISE probe:
Locate the small plastic bottle labeled with green tape. Rinse the bottle with distilled water. Fill the bottle three-quarters full with the Nitrate high standard solution, also labeled with green tape. Place the probe in this bottle, so that the tip of the probe does not touch the bottom, but so that the white dots (located just above the black rubber tip on the end of the probe) are completely submerged in the standard solution. Allow the probe to remain like this for at least thirty minutes.
Dissolved oxygen probe:
Carefully unscrew and remove the tip of the probe. Do not touch the membrane, or allow it to touch anything. Using the provided pipet, measure exactly one ml of the dissolved oxygen electrode filling solution. Place this one ml of the solution in the tip that was unscrewed from the probe. Gently screw the tip with the solution back onto the probe. Place the probe in a beaker filled with about 75 ml of distilled water and plug the probe into an active port. (The serial box connection on the computer in the lab works well. Be sure the green light on the back of the serial box is on, indicating that the serial box connection is receiving power. The probe has to be receiving power to effectively soak.)
Developed by Emily Blackwell with assistance from Dr. Mark Lassiter
This procedure is a modified version of the procedures for
:
| Vernier LabPro Probes | Extech Economy Oyster pH Meter Kit |
| Vernier Software & Technology | Extech Instruments Corporation |
| 13979 S.W. Millikan Way | 335 Bear Hill Road |
| Beaverton, OR 97005-2886 | Waltham, MA 02154 |
| www.vernier.com | Fax: (617) 890-7864 |
Site prepared by Mark Lassiter, 1/23/2002